Кафедра "Біотехнологія, біофізика та аналітична хімія"

Постійне посилання колекціїhttps://repository.kpi.kharkov.ua/handle/KhPI-Press/15

Офіційний сайт кафедри http://web.kpi.kharkov.ua/biotech

Кафедра "Бiотехнологiя, біофізика та аналiтична хiмiя" була створена у 1998 році на базі кафедри "Аналітична хімія", яка у 1940 році була виділена з кафедри хімії в самостійну кафедру. Ініціатива створення кафедри належить доктору технічних наук, професору Миколі Федосовичу Клещеву.

Кафедра входить до складу Навчально-наукового інституту хімічних технологій та інженерії Національного технічного університету "Харківський політехнічний інститут". Кафедра провадить освітню, методичну та наукову діяльність у галузі знань "Хімічна та біоінженерія". Крім теоретичних основ біотехнології, велику увагу було приділяється контролю якості і сертифікації біотехнологічної продукції.

У складі науково-педагогічного колективу кафедри працюють: 2 доктора наук: 1 – технічних, 1 – фармацевтичних; 8 кандидатів наук: 3 – біологічних, 5 – технічних; 2 співробітника мають звання професора, 6 – доцента.

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Anomalous polaritonic luminescence from rare-gas solids
(2016) Ogurtsov, A. N.; Kleshchev, N. F.; Bliznjuk, O. N.
Anomalous polaritonic luminescence from solid xenon
(2014) Ogurtsov, A. N.; Kleshchev, N. F.; Bliznjuk, O. N.
Catalytic ammonia oxidation to nitrogen (I) oxide
("East West" Association for Advanced Studies and Higher Education GmbH, 2015) Masalitina, Nataliya Yurevna; Savenkov, Anatoliy Sergeevich
The process of synthesis of nitrous oxide by low-temperature catalytical oxidation of NH has been investigated for organic synthesis. The investigation has been carried out by the stage separation approach with NH oxidation occurring in several reaction zones, which characterized by different catalytic conditions. The selectivity for N₂O was 92–92,5 % at the ammonia conversion of 98–99.5 % in the optimal temperature range.
Computer modeling of embryonic mortality at crioconservation
(Національна академія наук України; Інститут біохімії ім. О. В. Палладіна НАН України, 2016) Gorbunov, L. V.; Cherniavska, Ye. G.
The purpose of the research was to determine the regularities of influence of mammalian embryos heterogeneity and effectiveness of cryoconservation steps on their viability by using the developed simulation model. The model is based on analytical expressions that reflect the main causes of embryonic mortality during in vitro and in vivo cultivation, crioconservation and embryo transplantation. Reduction of viability depends on a set of biological factors such as the animal special, donor and recipient state, quality of embryos, and of technological ones such as the efficiency of cryopreservation method, and embryo transplantation. Fulfilled computer experiment showed, that divergence of embryos viability depending on biological parameters variations changes in a range from 0 to 100%, whereas efficiency index of chosen technology has an inaccuracy about 1%. The comparative analysis of alternative technologies of embryos cryopreservation showed the maximum efficiency of stages of use of the cryoprotectant, freezing regime and in vitro and in vivo cultivation of biological object. The application of computer modeling gives an opportunity to reduce the range of embryos viability results, obtained in different experiments is many times, thereby to shorten the time, monetary costs and the slaughter of laboratory animals in obtaining reliable results.
Correction of technological characteristics of proteinfat mixture by expanding the component composition
(РC Tесhnology сеntеr, 2018) Bochkarev, Sergiy; Varankina, Oleksandra; Bielykh, Iryna; Samoilenko, Serhii; Zviahintseva, Oksana
The object of research is the technological characteristics of the protein-fat mixture of increased nutritional value, depending on the addition of vegetable oil as a component. The protein-fat mixture is a mixture of dried, crushed flax seeds – 60.00±3.00 %, sesame seeds – 25.00±1.25 % and ground sunflower seeds – 15.00±0.75 % by weight of the seed mixture. One of the most problematic places in the production technology of this product is the fine grinding of raw materials, as well as the removal of the mixture from the chopper chamber due to the high viscosity of the mass. In the course of research, it is reasonable to expand the component composition of the protein-fat mixture of increased nutritional value by adding vegetable oil based on modeling and rationalizing the technological characteristics of the product. The following technological indicators of the product are identified in this research, as the effective viscosity and the separation degree of the mixture, depending on the mass fraction of added vegetable oil. Rational amount of vegetable oil in the formulation of protein-fat mixture of high nutritional value is proved. Compared with the production of protein-fat mixture without the introduction of this component, the proposed technological solution provides the ability to overcome difficulties in fine grinding of seeds, as well as the removal of the resulting paste from the chopper chamber. It is proved the necessary to use 15 % vegetable oil to obtain a protein-fat mixture with an effective viscosity and resistance to separation in accordance with the requirements of production. According to the content of flax seeds in the protein-fat mixture is 51.00±2.50 %, sesame seeds – 21.25±1.00 %, sunflower seeds – 12.75±0.60 %. The effective viscosity of the resulting protein-fat mixture is 16.0±1.5 Pa·s, and the degree of product separation is 0.20±0.01 %. Vegetable oil as a component of the protein-fat mixture improves its technological characteristics, which, in turn, simplifies the process of dosing and packaging the resulting product.
Cosmetic products containing probiotics: trends and issues
(Odesa I. I. Mechnikov National University, 2024) Putyatin, B.
Probiotics, defined as “live microorganisms that, when present in sufficient quantities, confer a health benefit on the host,” are becoming increasingly in demand in the market. However, many products labeled as probiotics do not meet these characteristics. In recent years, the cosmetics industry has seen an increase in the number of products claiming to be probiotic. Although probiotics may have several potential applications in personal care products, it is still necessary to ensure proper manufacturing practices to ensure that consumers actually receive probiotic products.
Crystal size effect in polaritonic luminescence from atomic cryocrystals
(Hyogo University, 2019) Ogurtsov, Alexander; Bliznjuk, O. N.; Masalitina, Nataliia
Development of rational technology for sodium glyceroxide obtaining
(Технологічний центр, 2022) Korchak, Mykola; Bliznjuk, O. N.; Nekrasov, Serhii; Gavrish, Tatiana; Petrova, Olena; Shevchuk, Natalia; Strikha, Liudmyla; Kostyrkin, Oleg; Semenov, Evgeny; Saveliev, Dmytro
The process of sodium glyceroxide obtaining by the reaction of glycerol and sodium hydroxide in the form of an aqueous solution was investigated. Glycerol salts (metal glyceroxides) are important components in the synthesis of many compounds. Glyceroxides are used in the chemical industry, construction, medical practice, etc. Glyceroxides of alkali metals are used in the production of modified fats and biodiesel fuel. P.a.-grade glycerol (CAS Number 56-81-5) was used with a mass fraction of the main substance of 99.5 %. The parameters of sodium hydroxide (CAS Number 1310-73-2) were studied: the mass fraction of the main substance is 98.0 %, the mass fraction of sodium carbonate is 0.5%. Rational conditions for sodium glyceroxide obtaining were determined: temperature (145 °C) and concentration of sodium hydroxide solution (65 %). Under these conditions, the mass fraction of the main substance in the product was 80 %. The melting point (72 °C) and mass fraction of moisture (0.3 %) in sodium glyceroxide were determined. The catalytic activity of the product in the process of transesterification of palm olein was tested. The increase in the melting point of palm olein was 15 °C. Under similar conditions of using potassium glyceroxide with a mass fraction of the main substance of 75.77 %, the increase in the melting point is 12.1 °C. This indicates an increase in the efficiency of the transesterification process using sodium glyceroxide obtained by the developed technology. The research results make it possible to produce sodium glyceroxide under rational conditions with a high mass fraction of the main substance at enterprises that use metal glyceroxides as a production component or commercial product. The determined rational conditions will make it possible to effectively use the company’s resources and predict the quality of the final product. Досліджено процес одержання натрій гліцерату шляхом реагування гліцерину та натрій гідроксиду у вигляді водного розчину. Солі гліцерину (гліцерати металів) є важливими складовими у процесах синтезу багатьох сполук. Гліцерати застосовуються у хімічній промисловості, будівництві, медичній практиці тощо. Гліцерати лужних металів використовуються у виробництві модифікованих жирів та біодизельного пального. Застосовано гліцерин (CAS Number 56-81-5) кваліфікації ч. д. а. з масовою часткою основної речовини 99,5 %. Досліджено показники натрій гідроксиду (CAS Number 1310-73-2): масова частка основної речовини – 98,0 %, масова частка натрій карбонату – 0,5 %. Встановлено раціональні умови отримання натрій гліцерату: температуру (145 °C) та концентрацію розчину натрій гідроксиду (65 %). За цих умов масова частка основної речовини в продукті склала 80 %. В натрій гліцераті визначено температуру плавлення (72 °C) та масову частку вологи (0,3 %). Перевірено каталітичну активність продукту у процесі переетерифікування олеїну пальмового. Підвищення температури плавлення олеїну пальмового склало 15 °C. За аналогічних умов використання калій гліцерату з масовою часткою основної речовини 75,77 % підвищення температури плавлення становить 12,1 °C. Це свідчить про підвищення ефективності процесу переетерифікування з використанням натрій гліцерату, отриманого за розробленою технологією. Результати досліджень дають можливість виробляти натрій гліцерат за раціональних умов з високою масовою часткою основної речовини на підприємствах, де використовують гліцерати металів як складову виробництва або товарний продукт. Встановлені раціональні умови дозволять ефективно використовувати ресурси підприємства та прогнозувати якість кінцевого продукту.
Development of safe technology of obtaining fatty acid monoglycerides using a new catalyst
(ПП "Технологічний Центр", 2022) Bliznjuk, O. N.; Masalitina, N. Yu.; Mezentseva, Iryna; Novozhylova, Tetiana; Korchak, Mykola; Haliasnyi, Ivan; Gavrish, Tatiana; Fomina, Iryna; Khalil, Viktoriya; Nikitchenko, Olga
Fatty acid monoglycerides are a valuable component of the products of various industries. The emulsifying ability of monoglycerides is used in cosmetic, pharmaceutical, and food production. The process of fatty acid monoglycerides obtaining by the reaction of vegetable hydrogenated fat (salomas) with glycerol (glycerolysis method) has been studied. Potassium glycerate is used as a catalyst, which is characterized by high efficiency and safety of production and use. A feature of the work is the study of the dependence of the yield and melting point of monoglycerides on the technological parameters of glycerolysis. As a raw material, hydrogenated refined fat according to DSTU 5040 (CAS Number 68334-28-1) was used: melting point – 48 °C, mass fraction of moisture and volatile substances – 0.08 %, acid value – 0.25 mg KOH/g, peroxide value – 2.8 ½ O mmol/kg. In all experiments, the glycerolysis temperature was 180 °C, the catalyst concentration – 0.5 % in terms of metal. Rational conditions for glycerolysis were determined: duration (90 min.) and glycerol concentration (50 %). Under these conditions, the monoglycerides yield was 32.9 %, melting point – 61.5 °C. The mass fraction of free glycerol in monoglycerides was 1.0 %, acid value – 2.2 mg KOH/g. The efficiency of monoglycerides obtaining using potassium hydroxide and glycerol mixture as a catalyst under certain rational conditions has been studied. The monoglycerides yield of 30.1 %, melting point of 59 °C were obtained. Therefore, the use of potassium glycerate catalyst is more efficient. The results of the study make it possible to improve the technology for the production of fatty acid monoglycerides using a new catalyst and use resources rationally.
Eisenia fetida (lumbricimorpha: lumbricidae), як біотрансформатор вмісту основних груп катіонів та аніонів субстрату
(Наука-сервіс, 2010) Лутохін, С. Б.; Ларінцева, Надія Вікторівна
Energy loss and inelastic scattering of photoelectrons in N₂ doped solid Kr
(al-Farabi Kazakh National university, 2014) Ogurtsov, A. N.; Bliznjuk, O. N.; Masalitina, N. Yu.
Evaluation of quality and composition of essential oils in composition of cosmetic products
(Nauka i studia, 2020) Levchuk, I. V.; Holubets, O. V.; Putiatin, B. V.; Kunitsia, E. V.
The article is concerned with complex approach to the evaluation of quality and composition of essential oils on the example of tea tree oil and lavender oil. Efficacy of a cosmetic product depends directly on the quality of raw materials used. Only qualitative, natural essential oil can provide a warranty that the product, in which composition this oil is used, meets al labeled characteristics and functional properties. The article corroborates practicability of complex approach to the evaluation of quality and composition of essential oils. The results obtained suggest the necessity of more stringent quality control of essential oils during production of perfumes and cosmetic products.
Evidence of defect phase formation in photoirradiated solid Xe: Steady-state kinetic study
(2006) Masalitina, N. Yu.; Bliznjuk, O. N.; Ogurtsov, A. N.
Experiment planning at the pharmaceutical development of liposomal cytostatics
(Національний фармацевтичний університет, 2017) Stadnichenko, A. V.; Krasnopolsky, Yu. M.; Yarnykh, T. G.
At present, there is an increasing interest in developing new ways of drug delivery and targeted therapy, using nanotechnology and nanomaterials. Aim – to study the order of carrying out of pharmaceutical development of liposoms with cytostatics. Propose the scope of the experiment to optimize the planned quality indicators and technological parameters. Materials and methods. Analysis of normative documents, scientific literature and also the results of previous personal experimental studies, which became the basis for determining the methodology for the creation of liposomal drugs based on oxaliplatin and irinotecan. Lipids manufactured by Lipoid, Germany, were used to make liposomes. The lipid film was produced on a Buchi 210 rotary evaporator with a vacuum controller, at a residual pressure of 0.02 atm. For homogenization, a high pressure extrusion method was used, which was carried out on a Microfluidiser M-110P (Microfluidics, USA). Results and discussion. With the development of the pharmaceutical industry, there is a growing interest to the use of nanotechnology and nanomaterials. One of the practical implementation of nanotechnology is liposomes with cytostatics. Concentration of the active substance; pH and salt API; method of loading for API into liposomes; lipid to lipid ratio, lipids concentration; particle size and internal volume; lipid solubility in the step of lipid film preparation; stability testing of of finished products are factors that need to be studied and solved for the successful implementation of the development. Planning an experiment in the pharmaceutical development of liposomal oxaliplatin and liposomal irinotecan are complex studyes with using the principles of Quality by Design (QbD). Conclusions. The requirements of normative documentation for creating liposomal forms of medicinal products are considered. At pharmaceutical development it is necessary to use the complex approach as the majority of the put questions can not be solved separately. An experiment planning system for the pharmaceutical development of liposomal preparations of irinotecan and oxaliplatin is proposed.
Experimental study of liposomal docetaxel analysis of docetaxel incorporation and stability
(Morion LLC, 2017) Krasnopolsky, Yu. M.; Dudnichenko, А. S.
The article presents the results of developing the composition and technology of obtaining the liposomal form of docetaxel. The effect of the phospholipid composition of the membrane, ionic strength, pH, temperature, cryoprotectant type, and other factors on the stability of liposomes and the docetaxel incorporation has been considered. Results: Reduction of toxicity of the liposomal form of docetaxel (LD50 – 137 ± 7.7 mg/kg) was found in comparison with its free form (LD50 – 101 ± 6.3 mg/kg). Preservation of nanosize particle after lyophilization has been shown. Conclusions: As a result of the studies, the optimal composition and technological scheme for obtaining liposomes containing docetaxel have been developed allowing large-scale production of docetaxel in liposomal form.
The influence of complex liposomal antioxidant preparations on biological oxidative stress markers in ischemic heart disease
(Publishing center ESCBM, Česká republika, 2020) Pylypenko, D. M.; Gorbach, T. V.; Krasnopolsky, Yu. M.
Influence of heart function parameters – heart rate and heart rate variability on the course of chronic heart failure in patients with arterial hypertension
(Nauka i studia, Przemysl, 2018) Ilchenko (Bielykh), I. A.; Karamian, A. A.
Kinetic analysis of sample degradation progress curves
(2005) Ogurtsov, A. N.; Masalitina, N. Yu.; Bliznjuk, O. N.
Kinetic modeling of the catalytic ammonia oxidation to N₂O
(Український державний хіміко-технологічний університет, 2017) Bliznjuk, O. N.; Ogurtsov, A. N.; Savenkov, A. S.; Masalitina, N. Yu.
Low temperature oxidation of ammonia to N₂O over mixed oxide catalysts
(2017) Bliznjuk, O. N.; Ogurtsov, A.; Masalitina, N.
The study is devoted to development of technology of catalytic ammonia oxidation to nitrogen(I) oxide. The process of low-temperature oxidation of ammonium on oxidizing catalysts has been investigated for production of nitrogen(I) oxide to be used for medical purposes and organic synthesis. Mixed oxide catalysts under various technological parameters were investigated. Experimentally validated concepts became the basis for creation the technological process of ammonia oxidation. Technological scheme of nitrogen(I) oxide production for organic synthesis was developed. The developed technological process is pro-posed for industrial implementation and appropriate output data are provided for industrial design.