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Постійне посилання на розділhttps://repository.kpi.kharkov.ua/handle/KhPI-Press/35393
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Документ Study of antioxidant activity of liposomal forms of quercetin and curcumin in ischemic heart disease(Termedia Publishing House, Poland, 2020) Pylypenko, D. M.; Gorbach, T. V.; Krasnopolsky, Yu. M.Quercetin and curcumin are plant polyphenolic antioxidants with proven pharmacological efficacy. Their use is, however, limited due to low bioavailability and oral administration route. The encapsulation of lipophilic compounds in liposomes enables to increase their bioavailability and to create an injectable form. The present study aimed to comparatively investigate the antioxidant activity of a complex liposomal preparation containing two lipophilic antioxidants (quercetin and curcumin) and their monopreparations in liposomal forms. This study was conducted on Wistar line rats with experimental model of ischemic heart disease. Oxidative stress markers such as total antioxidant activity, malondialdehyde, and peroxidized proteins were analyzed in blood serum and cardiac tissue. Ischemic heart disease is accompanied with lipid peroxidation and changes in the activity of the antioxidant system. The obtained results demonstrated the antioxidant activity of monopreparations of curcumin and quercetin and their complex in liposomal forms. Quercetin and curcumin showed different antioxidant activities in terms of oxidative stress markers. The complex of the two antioxidants showed the synergistic effect of their lipophilic compounds in liposomal forms, which led to the normalization of test parameters according to the level of the control sample.Документ A Study of Oxidative Stress Markers when Using the Liposomal Antioxidant Complex(Hellenic Pharmaceutical Society, 2019) Pylypenko, D. M.; Gorbach, T. V.; Katsai, O. G.; Grigoryeva, A. S.; Krasnopolsky, Yu. M.The aim of the study was to create the complex liposomal preparation containing Coenzyme Q10 and Quercetin and learn its antioxidant activity. The liposomes were obtained the lipid film method followed by emulsification with PBS, extrusion and sterilizing filtration. The emulsion was lyophilized with lactose as a cryoprotectant. The ratio of Coenzyme Q10 and Quercetin in complex liposomal composition was 1:1. The particles size and the degree of inclusion were determined. Pharmacological study was carried out on Wistar line rats with experimental ischemic heart disease model. The samples of complex liposomal composition and monocompositions of Coenzyme Q10 and Quercetin were intravenously administered daily for 5 days at a dose of 10 mg/kg. Oxidative stress markers (levels of malondialdehyde, conjugated dienes, sulfhydryl groups, protein peroxidation, enzyme superoxide dismutase catalase and total antioxidant activity) were analyzed in blood serum and cardiac tissue of the rats. The obtained complex composition was characterized by a particle size of up to 220 nm and at least 90.0 % of the included Coenzyme Q10 and Quercetin. A decrease in the level of lipid oxidation products and an increase in the activity of antioxidant system using the complex liposomal preparation containing Coenzyme Q10 and Quercetin compared to its monocompositions in liposomal form were shown.