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  • Ескіз
    Документ
    Influence of structure 3,5,7,3',4'–Pentahydroxyflavone-based polymer films on their optical transparency
    (2017) Mishurov, Dmytro; Voronkin, Andrii; Roshal, Alexander; Bogatyrenko, Sergey
    Optical properties and morphology of polymer films based on diglycidyl ether of bis-phenol A and on natural compound 3,5,7,30 ,40 epentahydroxyflavone (quercetin) using UVevis spectroscopy, scanning electron microscopy and powder X-ray diffraction methods have been investigated. The influence of ordering degree of the polymers, flexibility of their chains, intensity of intermolecular interaction between the quercetine moieties on the polymers' morphology was discussed. It is shown that all the quercetine-based polymer films are transparent at visible and near infrared regions beginning from 420 to 450 nm. Low optical transparency in the short-wavelength region can be explained the self-absorption of quercetin moieties in the polymer chains
  • Ескіз
    Документ
    Possibility of computer experiment in study of animal spermatozoa heterogeneity
    (Національна академія наук України; Інститут біохімії ім. О. В. Палладіна НАН України, 2016) Gorbunov, L. V.; Mazharova, Y. M.
    A simulation model for evaluating the survival and fertilizing capacity of animal spermatozoa was developed, taking into account the initial condition of the sperm and the effectiveness of cryopreservation stages. The model is based on an analytical expression that reflects the main reasons for the survival of reproductive cells in onto-, techno- and phylogenesis. The decrease in spermatozoa resistance depends on a number of biological factors — the animal species, physiological conditions of sperm donor and recipient, the ejaculate quality, and technological factors — the effectiveness of the methods of cell cryopreservation and egg insemination. The discrepancy between the results of cell motility obtained by calculation and experimental methods amounted to less than 2% as a result of our own experiments and to less than 5% for the data taken from literature. A feature of the model is the complete independence of the effectiveness of studied techniques from the heterogeneity of animal sperm. The conducted computer experiment showed that the difference between the values of initial motility and fertilizing capacity of sperm varies from 50 to 100% depending on the difference of biological parameters, while the index of the effectiveness of selected technique creates an error of about 1%. Comparative analysis of alternative technologies of spermatozoa cryopreservation showed the maximum efficiency of the stages of cryoprotectant use, freeze mode, survival and fertilizing capacity of the object. The use of computer modeling allows to greatly reduce the spread in spermatozoa preservation values that were obtained in different experiments, and thus to reduce the time and costs it takes to obtain reliable results.