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Постійне посилання на розділhttps://repository.kpi.kharkov.ua/handle/KhPI-Press/35393
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3 результатів
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Документ Preparation and in-vivo evaluation of cytochrome-C-containing liposomes(GOVI Verlag, Germany, 2017) Katsai, O. G.; Ruban, O. A.; Krasnopolsky, Yu. M.This study investigates the development of a method for obtaining cytochrome C-containing liposomes (LS-Cyt), and evaluates their stability and specific activity. LS-Cyt were intended for the therapy of ophthalmic diseases. LS-Cyt were prepared by high pressure homogenization technique and lyophilized to obtain freeze-dried LS-Cyt. It was proposed to use anionic phospholipid- dipalmitoylphosphatidylglycerol (DPPG-Na) and phosphatidylcholine (PC) in a nanoparticulate composition. Were investigated various concentrations of lactose and trehalose as cryoprotectants. Samples with a lactose concentration of 6% showed the best results in terms of the emulsion formation time, encapsulation and preservation of nanosize. The main technological parameters for the obtained freeze-dried LS-Cyt were encapsulation of no less than 95% of cytochrome C (Cyt C), particle size of 140-170 nm, pH of 6.85±0.1, osmolarity of 330±3 mOsmol/kg, a lysophosphatidylcholine content (LPC) of 0.65±0.05 % of the total of lipids. Stability of the freeze-dried LS-Cyt during storage was established. The freeze-dried LS-Cyt was kept for 1 year in a light protected place at the temperature of -15 °C. No changes in the composition of LS-Cyt samples were detected over the observation period. Preclinical in-vivo research was conducted, namely the evaluation of specific activity on the model of the penetrating corneal injury. It was established that use of LS-Cyt contributes to a more rapid process of tissue regeneration and reduction of the inflammatory response in comparison with a non-liposomal dosage form.Документ A Study of Oxidative Stress Markers when Using the Liposomal Antioxidant Complex(Hellenic Pharmaceutical Society, 2019) Pylypenko, D. M.; Gorbach, T. V.; Katsai, O. G.; Grigoryeva, A. S.; Krasnopolsky, Yu. M.The aim of the study was to create the complex liposomal preparation containing Coenzyme Q10 and Quercetin and learn its antioxidant activity. The liposomes were obtained the lipid film method followed by emulsification with PBS, extrusion and sterilizing filtration. The emulsion was lyophilized with lactose as a cryoprotectant. The ratio of Coenzyme Q10 and Quercetin in complex liposomal composition was 1:1. The particles size and the degree of inclusion were determined. Pharmacological study was carried out on Wistar line rats with experimental ischemic heart disease model. The samples of complex liposomal composition and monocompositions of Coenzyme Q10 and Quercetin were intravenously administered daily for 5 days at a dose of 10 mg/kg. Oxidative stress markers (levels of malondialdehyde, conjugated dienes, sulfhydryl groups, protein peroxidation, enzyme superoxide dismutase catalase and total antioxidant activity) were analyzed in blood serum and cardiac tissue of the rats. The obtained complex composition was characterized by a particle size of up to 220 nm and at least 90.0 % of the included Coenzyme Q10 and Quercetin. A decrease in the level of lipid oxidation products and an increase in the activity of antioxidant system using the complex liposomal preparation containing Coenzyme Q10 and Quercetin compared to its monocompositions in liposomal form were shown.Документ "Quality-by-Design" approach to the development of a dosage form for the liposomal delivery system of cytochrome С(Hellenic Pharmaceutical Society, 2018) Katsai, O. G.; Ruban, O. A.; Krasnopolsky, Yu. M.Cytochrome C (Cyt C) is a natural catalyst for cellular respiration. Aqueous solutions of Cyt C are used in cardiology, neurology and ophthalmology, where they manifests a high activity towards free radicals and bind aggressive molecules of oxidants. The aim of the study was to develop and optimize the preparation of freeze-dried cytochrome C-containing liposomes (FD-Cyt-LS) using the Quality by Design (QbD) approach and to compare the stability of aqueous Cyt-LS and FD-Cyt-LS. In the case of freeze-dried Cyt-LS (FD-Cyt-LS), the influence of cryoprotectants type and concentration, as well as secondary drying temperature, which ensure integrity of liposomal membrane during lyophilization and preservation throughout the shelf life were studied. Lactose with a concentration of 6% was established to be the optimal type of cryoprotectant. At the lyophilization programme selected the most preferable secondary drying temperature value was 30°C. The necessity to choose the form of lyophilizate for solution preparation was shown with the example of stability evaluation of aqueous Cyt-LS and FD-Cyt-LS. During the observation period, aqueous Cyt-LS shows the growth of degradation products of free fatty acids and lysophosphatidylcholine (LPC) by 202.5% and 409.7% respectively, compared with the reference values, as well as the decrease of the content of phosphatidylcholine (РС) and dipalmitoylphosphatidylcholine (DPPG- Na) by 1.5 and 1.0%, respectively. At the same time, no significant changes in the composition of FD-Cyt-LS samples were observed.