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Документ Changes in cell membranes of white blood cells treated with a common food additive E407a(2021) Tkachenko, A. S.; Onishchenko, A.; Posokhov, Ye. O.; Roshal, Alexander; Myasoedov, Valeriy; Nakonechna, OksanaChanges are detected in the spectra of the fluorescent probe bound to rat WBCs treated with the solutions of E407a of various concentrations in comparison with the corresponding spectra of the probe incubated with the untreated leukocytes. The decrease in polarity and protondonor ability is observed in the lipid membranes of leukocytes in the region, where the probe locates.Документ Experimental Evaluation of Food-Grade Semi-Refined Carrageenan Toxicity(2021) Pogozhykh, Denys; Posokhov, Ye. O.; Myasoedov, Valeriy; Gubina-Vakulyck, Galina; Chumachenko, Tetyana; Knigavko, Oleksandr; Polikarpova, Hanna; Kalashnyk-Vakulenko, Yuliia; Sharashydze, Ketino; Nakonechna, Oksana; Prokopyuk, Volodymyr; Onishchenko, Anatolii; Tkachenko, AntonThe safety of food additives E407 and E407a has raised concerns in the scientific community. Thus, this study aims to assess the local and systemic toxic effects of the common food additive E407a in rats orally exposed to it for two weeks. Complex evaluations of the effects of semi-refined carrageenan (E407a) on rats upon oral exposure were performed. Local effects of E407a on the intestine were analyzed using routine histological stains and CD68 immunostaining. Furthermore, circulating levels of inflammatory markers were assessed. A fluorescent probe O1O (2-(20-OH-phenyl)-5-phenyl-1,3-oxazole) was used for evaluating the state of leukocyte cell membranes. Cell death modes of leukocytes were analyzed by flow cytometry using Annexin V and 7-aminoactinomycin D staining. Oral administration of the common food additive E407a was found to be associated with altered small and large intestinal morphology, infiltration of the lamina propria in the small intestine with macrophages (CD68+ cells), high systemic levels of inflammation markers, and changes in the lipid order of the phospholipid bilayer in the cell membranes of leukocytes, alongside the activation of their apoptosis. Our findings suggest that oral exposure to E407a through rats results in the development of intestinal inflammation.Документ A study of cell membranes in nasal epithelial cells from patients with chronic rhinosinusitis with nasal polyps by means of a fluorescent probe(2019) Onishchenko, A. I.; Tkachenko, A. S.; Nakonechna, O. A.; Korniyenko, Y. M.; Ryshchenko, I. M.; Tsygankov, A. V.; Posokhov, Ye. O.Aim. To assess the state of membranes in nasal epithelial cells obtained from the patients with chronic rhinosinusitis with nasal polyps (CRSwNP) with the help of the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole. Methods. The state of membrane phospholipid bilayer in suspensions of nasal epithelial cells isolated from ten patients with CRSwNP was evaluated using the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole that reacts on the physico-chemical properties of its microenvironment. Changes in fluorescence spectra were determined using a Thermo Scientific Lumina fluorescence spectrometer (Thermo Fisher Scientific) 1 hour after the addition of the probe to nasal epithelial cell suspensions. Results. CRSwNP was found to be associated with a higher rate of nasal epithelial cell membrane hydration in the region of phospholipid glycerol moiety, carbonyl groups and aliphatic chains of fatty acids attached to the carbonyl groups. Conclusion. Our findings suggest that CRSwNP is accompanied by the elevated hydration rate of the most polar region, namely polar heads of phospholipids of nasal epithelial cell membranes.Документ Синтез та дослідження похідних карбонових кислот – потенційних біологічно активних речовин. 2. Дослідження біологічної активності деяких похідних 4-морфолінонафталіміду в якості флуоресцентних зондів(НТУ "ХПІ", 2014) Дістанов, Віталій Баламірович; Рошаль, О. Д.; Дюбко, Т. С.; Фалалєєва, Тетяна ВасилівнаВ даній статті розглянуті питання можливості використання похідних 4-мофолінонафталевої кислоти в якості флуоресцентних зондів для виявлення деяких захворювань на ранній стадії. Багато яких похідних цієї кислоти мають різноманітну біологічну активність. В залежності від присутності в структурі молекули замісників різної природи (електронодонорні або електроноакцепторні) змінюється вплив на можливу взаємодію речовини з організмом людини. Розглянуті деякі аспекти до цього підходу.