Methodical guidelines for conducting laboratory classes in the academic discipline "Industrial technologies of microbial synthesis"

Abstract

The organization of any microbiological synthesis of practically important substances begins with the study of the physiology of the producer (the nature of the producer's nutrition, the influence of external factors on the state of cells and populations). These issues are clarified by optimizing the process under study. The basis for this is the cultivation of microorganisms in controlled and managed conditions. Ultimately, the composition of the media and the cultivation regime are established, the most effective and economically beneficial. The next stage is to scale up the process and test it in industrial conditions. In this case, there is a gradual transition from the cultivation of microorganisms in flasks (obtaining seed material) to growing in laboratory fermenters and then in large-scale industrial installations. The absence of extraneous microorganisms and bacteriophages is also controlled, and conditions are created to maintain high activity of the producer. The purpose of laboratory work is to deepen and consolidate students' knowledge and skills that provide a general approach to the optimal cultivation of industrial biotechnology products, protein biosynthesis and a comprehensive assessment of the quality of the final product. The results of the research are described in the calculation work, conclusions are drawn and the work is defended. In the process of performing laboratory work, students investigate how changes in the composition of nutrient media and cultivation conditions affect the yield and quality of the protein product. The acquired knowledge and skills will be useful when carrying out technological processes in biotechnological production.