Кафедра "Біотехнологія, біофізика та аналітична хімія"

Постійне посилання колекціїhttps://repository.kpi.kharkov.ua/handle/KhPI-Press/15

Офіційний сайт кафедри http://web.kpi.kharkov.ua/biotech

Кафедра "Бiотехнологiя, біофізика та аналiтична хiмiя" була створена у 1998 році на базі кафедри "Аналітична хімія", яка у 1940 році була виділена з кафедри хімії в самостійну кафедру. Ініціатива створення кафедри належить доктору технічних наук, професору Миколі Федосовичу Клещеву.

Кафедра входить до складу Навчально-наукового інституту хімічних технологій та інженерії Національного технічного університету "Харківський політехнічний інститут". Кафедра провадить освітню, методичну та наукову діяльність у галузі знань "Хімічна та біоінженерія". Крім теоретичних основ біотехнології, велику увагу було приділяється контролю якості і сертифікації біотехнологічної продукції.

У складі науково-педагогічного колективу кафедри працюють: 2 доктора наук: 1 – технічних, 1 – фармацевтичних; 8 кандидатів наук: 3 – біологічних, 5 – технічних; 2 співробітника мають звання професора, 6 – доцента.

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Фільтри

2016 - 20172

Налаштування

Автор: search.filters.author.Stadnichenko, O. V.

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  • Ескіз
    Документ
    The study of the lipid membrane charge effect when creating liposomes with oxaliplatin
    (Національний фармацевтичний університет, 2016) Stadnichenko, O. V.; Krasnopolsky, Yu. M.; Yarnykh, T. G.
    The fight against cancer diseases is one of the most urgent problems of modern pharmacy. One of the basic standards of treatment is surgery in order to remove tumours with chemotherapeutic agents for suppression of disease manifestations. One of the ways for reducing toxicity of cytostatics is their incorporation into liposomes – nanoparticles composed of the lipid bilayer surrounding the internal cavity with the aqueous medium. Oxaliplatin is a platinum-containing chemotherapeutic agent of the 3-rd generation used as monotherapy or in combination with other drugs. When creating liposomal drugs the beginning of the work is associated with the study of the composition of the lipid membrane. The aim of the work is to study the effect of the lipid membrane charge when creating liposomes with oxaliplatin. Four types of differently charged lipid membranes for liposomal oxaliplatin formulation have been tested. Liposomes were formed by the lipid layer method with further high pressure homogenization. As a criterion the encapsulation degree was used. The highest encapsulation degree has been determined in negatively charged liposomes with the lipid membrane modified by dipalmitoyl phosphatidylglycerol (DPPG).
  • Ескіз
    Документ
    The study of liophilization parameters in the liposomal irinotecan development
    (Національний фармацевтичний університет, 2017) Stadnichenko, O. V.; Krasnopolsky, Yu. M.; Yarnykh, T. G.
    The creation of the liposomal irinotecan is one of the main ways to reduce toxicity and increase the effectiveness of chemotherapy. Lyophilization makes it possible to obtain a product with a guaranteed stability of the size and encapsulation efficiency. Aim. To optimize the content of the cryoprotector in the liposomal irinotecan, and develop lyophilization parameters to produce liposomes with the maximum encapsulation of irinotecan in them, alongside while maintaining the nanosize. Materials and methods. Egg phosphatidylcholine from Lipoid (Germany) was used for preparation of liposomes. Lyophilization was carried out in a Quarco device (PR China). The encapsulation degree was determined on a Shimadzu LC-20 instrument (Japan) by HPLC method developed earlier. Results and discussion. The optimal content of the cryoprotector – trehalose dihydrate has been studied. It has been found that the optimal content of trehalose dihydrate is 8 % (w/w). The modes of the product lyophilization have been studied. The secondary drying temperature in the range of 10-20 °C has been determined. At the secondary drying temperature of 10 °C the residual moisture content was 5-8 %, which was beyond the target range. At 20 °C the water content in the lyophilizate was 0.5-0.8 %, the loss of encapsulation was up to 20 %. The mode of drying at 15 °C was optimal, while the residual water content in the lyophilizate was 1.5-2.8 %, the loss of encapsulation was 13 %, the size of the liposomes after lyophilization and rehydration did not change significantly compared to the initial one. Сonclusions. As a result of the studies, liposomes with irinotecan have been obtained. The content of trehalose dihydrate as a cryoprotector in the range of 4-10 % has been studied. It has been shown that the optimum content of trehalose dihydrate is 8 % (w/w); moreover, the encapsulation decrease in lyophilization is 13 %. The modes of the liposomal irinotecan lyophilization have been studied at the final drying temperature of 10, 15 and 20 °C. It has been found that the optimum final drying temperature is 15 °C.